《植物生理学报》 2014, 50(8): 1243-1247

6-BA和GA₃对牡丹叶片衰老过程中生理特性的影响

李金航¹, 郭丽丽¹, 孔祥生^1,*, 张淑玲², 闫臻¹

¹河南科技大学农学院, 河南洛阳471003; ²洛阳国际牡丹园, 河南洛阳471003

通信作者：孔祥生；E-mail: kxsh55@163.com；Tel: 0379-64282253

摘　要：

以‘洛阳红’和‘胡红’为试材, 研究了牡丹衰老过程中喷施不同浓度的6-BA和GA₃对叶片净光合速率(P_n), 可溶性糖、可溶性蛋白和丙二醛(MDA)含量以及细胞膜相对透性的影响。结果表明: 7月30日喷施浓度100、200、300 mg•L^-1的6-BA或GA₃可以显著提高‘洛阳红’和‘胡红’叶片的P_n以及可溶性糖和可溶性蛋白含量, 明显抑制叶片中MDA的积累, 降低叶片细胞膜相对透性; 2种植物生长调节剂均以300 mg•L^-1处理效果最显著。8月10日喷施浓度为300 mg•L^-1的6-BA或GA₃, 可以有效延缓‘洛阳红’和‘胡红’叶片的衰老, 而低浓度的6-BA和GA₃处理效果不显著。

关键词：牡丹; 叶片衰老; 生理特性; 6-BA; GA₃

收稿：2014-05-26   修定：2014-07-14

资助：国家自然科学基金(31200468)。

Effects of 6-BA and GA₃ on Physiological Characteristics during Leaf Senescence of Paeonia suffruticosa

LI Jin-Hang¹, GUO Li-Li¹, KONG Xiang-Sheng^1,*, ZHANG Shu-Ling², YAN Zhen¹

¹College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471003, China; ²Luoyang International Peony Garden, Luoyang, Henan 471003, China

Corresponding author: KONG Xiang-Sheng; E-mail: kxsh55@163.com; Tel: 0379-64282253

Abstract:

In this study Paeonia suffruticosa ‘Luoyanghong’ and ‘Huhong’ were used as the test materials. Effects of different concentrations of 6-BA and GA₃ on physiological characteristics including net photosynthetic rate (P_n), soluble sugar, soluble protein and malondialdehyde (MDA) contents, and relative membrane permeability during leaf senescence were determined. The results showed that spraying 6-BA and GA₃ at concentrations of 100, 200 and 300 mg•L^-1 on July 30th could improve P_n, and soluble sugar and protein contents significantly, while MDA accumulation in leaves was inhibited obviously, and the relative cell membrane permeability was reduced. The appropriate concentration of the two kinds of growth regulator was 300 mg•L^-1. Spraying 6-BA or GA₃ at the concentration of 300 mg•L^-1 on August 10th could effectively delay leaf senescence of ‘Luoyanghong’ and ‘Huhong’, while 6-BA and GA₃ of lower concentrations showed no remarkable effect.

Key words: Paeonia suffruticosa; leaf senescence; physiological characteristics; 6-BA; GA₃